In a series of tweets, the USA leader appeared to lash out at the idea of a European army - which Mr Macron and German Chancellor Angela Merkel recently suggested could be a good idea "one day" - and demanded that France "pay for NATO".
Mr Trump returned to Washington from a weekend in Paris to commemorate the 100th anniversary of World War I, where strained relations between the American President and European allies was on full display.
Trump was referring to a speech Macron delivered over the weekend on the centenary of World War I in which the French president derided the perils of nationalism in a veiled critique of Trump's America First doctrine.
The two men had previously enjoyed a warm friendship, with Macron becoming the first world leader to be honored with a USA state visit since Trump's 2017 inauguration in April this year.
Trump lit into Macron Tuesday over his suggestion for a European defense force, French tariffs on USA wine and even Macron's approval ratings.
And looking ahead to his appearance in 'Avengers 4'
If you don't work in a school, you've probably seen the same thing on the subway or bus or anywhere in China, really. I long admired (and emulated) your kindness and patience and sense of humor when dealing with your public.
In his tweet on Tuesday, Trump again referenced France's spending, writing: "Pay for North Atlantic Treaty Organisation or not!"
'Secret Service said NO, too far from airport & big Paris shutdown.
Trump followed his bashing of Macron with an all-caps "make France great again" tweet.
Fresh from his trip to Paris where he offended much of Europe for not attending an important event because it was raining, President Donald Trump is going ballistic, attacking Emmanuel Macron.
And on Sunday, Trump arrived separately from the 60 other leaders at a World War I remembrance at the Arc de Triomphe.
Gilbert Collard, a politician who supported Le Pen's party, said that although he felt Trump was "absolutely right" in all his attacks on Macron, he was "absolutely wrong on what he says about French wine".
On Tuesday, Trump fired off a caustic series of tweets against his weekend host and renewed his frequent charge that America's European allies in North Atlantic Treaty Organisation spend too little on defence.
III basketball player suspended for ruthlessly elbowing opponent
On behalf of the MASCAC and Fitchburg State, we apologize to the Nichols College student-athlete, the team and institution". Full video of the game confirms that there were no previous physical altercations but does provide a bit more context.
Trump tweeted about a suggestion by French President Emmanuel Macron that Europe build up its militaries because the continent can no longer depend on the U.S. for defence. "Little reported-Fake News!" Trump tweeted. Macron also criticized Trump's vow to abandon the decades-long nuclear deal with Russian Federation.
The president was scheduled to pay tribute at the Aisne-Marne American Cemetery in Belleau, about 50 miles east of Paris with Melania.
Macron rejected nationalism in a speech on Sunday, in what many considered a rebuke of Trump.
GettyGerman Chancellor Angela Merkel and French President Emmanuel Macron lay a wreath at ceremony Donald Trump skipped due to rain.
"Very insulting, but perhaps Europe should first pay its fair share of North Atlantic Treaty Organisation, which the USA subsidizes greatly!" tweeted Trump.
He also slammed the French leader's low approval ratings and the country's high level of unemployment. "Not fair, must change!" he wrote.
Of course, Macron isn't the only French official calling for the creation of a "European army".
Teams search for bodies in California after wildfire claims 42 lives
Butte County Sheriff Honea said that some remains may not be found before residents start returning to their intact homes. Interior Secretary Ryan Zinke said he canceled a trip to Asia and will visit the fire zones Wednesday and Thursday.